Restoration of excitation-contraction coupling and slow calcium current in dysgenic muscle by dihydropyridine receptor complementary DNA

Nature. 1988 Nov 10;336(6195):134-9. doi: 10.1038/336134a0.


Microinjection of an expression plasmid that carries complementary DNA encoding the receptor for dihydropyridine calcium channel blockers of skeletal muscle restores both excitation-contraction coupling and slow calcium current in cultured skeletal muscle cells from mice with muscular dysgenesis. This suggests that the dihydropyridine receptor in the transverse tubule membrane of skeletal muscle functions both as the voltage sensor for excitation-contraction coupling and as the slow calcium channel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cells, Cultured
  • DNA / metabolism
  • Dihydropyridines / metabolism*
  • Mice
  • Muscle Contraction*
  • Muscular Diseases / genetics
  • Muscular Diseases / metabolism*
  • Mutation
  • Polymorphism, Restriction Fragment Length


  • Dihydropyridines
  • DNA
  • Calcium