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, 45 (19), 10941-10947

The FASTK Family of Proteins: Emerging Regulators of Mitochondrial RNA Biology


The FASTK Family of Proteins: Emerging Regulators of Mitochondrial RNA Biology

Alexis A Jourdain et al. Nucleic Acids Res.


The FASTK family proteins have recently emerged as key post-transcriptional regulators of mitochondrial gene expression. FASTK, the founding member and its homologs FASTKD1-5 are architecturally related RNA-binding proteins, each having a different function in the regulation of mitochondrial RNA biology, from mRNA processing and maturation to ribosome assembly and translation. In this review, we outline the structure, evolution and function of these FASTK proteins and discuss the individual role that each has in mitochondrial RNA biology. In addition, we highlight the aspects of FASTK research that still require more attention.


Figure 1.
Figure 1.
(A) Schematic representation of the FASTK family proteins. N: Amino-terminal domain. C: carboxy-terminal domain. The arrow indicates the internal translation start site in FASTK mRNA that generates mitoFASTK (B) Conservation of the FASTK family across evolution according to InterPro (12). Only proteins containing a FAST_1, FAST_2 and a RAP domain on the same polypeptide are reported. Redundant proteins from the database have been filtered out. (C) Proposed mechanism of action of the members of the FASTK family, as detailed in the main text. Ψ: pseudouridine. (D) (top) I-TASSER prediction of FASTK structure (40) and (bottom) crystal structure of a designed PPR protein (PDB ID: 4WSL) (41).

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