Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying blaIMI-Type Carbapenemase Genes in Enterobacter cloacae Complex Isolates in Singapore

Antimicrob Agents Chemother. 2017 Dec 21;62(1):e01542-17. doi: 10.1128/AAC.01542-17. Print 2018 Jan.

Abstract

Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine the flanking regions of blaIMI-type genes. Phylogenetic analysis of multilocus sequence typing (MLST) targets separated the isolates into 4 clusters. The blaIMI-type genes were all found on Xer-dependent integrative mobile elements (IMEX). The IMEX elements of 5 isolates were similar to those described in Canada, while the remainder were novel. Five isolates had IMEX elements lacking a resolvase and recombinase.

Keywords: antimicrobial resistance; carbapenemase; mobile genetic elements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Enterobacter cloacae / drug effects*
  • Enterobacter cloacae / genetics*
  • Enterobacter cloacae / isolation & purification
  • Genome, Bacterial / genetics
  • Humans
  • Interspersed Repetitive Sequences / genetics*
  • Multilocus Sequence Typing
  • Singapore
  • Whole Genome Sequencing
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase