Objective: To investigate the effect of low and high oxygen concentration on embryo development, pregnancy outcome and birth defects of in vitro fertilization-embryo transfer (IVF-ET).
Methods: According to the oxygen concentration of in vitro culture environment, the IVF-ET performed in the Women's Hospital, Zhejiang University School of Medicine during 2013 and 2015 were divided into low oxygen concentration group (n=2036, 5% O2) and high oxygen concentration group (n=4617, 20% O2). The rate of fertilization, good quality embryo, clinical pregnancy, ectopic pregnancy, abortion and birth defect were compared between two groups.
Results: The good quality embryo rate was significantly higher in the low oxygen concentration group (P<0.05). However, no significant differences were found between two groups in the rate of fertilization, clinical pregnancy, ectopic pregnancy, abortion and birth defect (all P>0.05).
Conclusions: Low oxygen environment may improve the potential of embryonic development, but its impact on pregnancy outcome and birth defect is not significant.
目的: 比较低氧浓度与高氧浓度培养对体外受精-胚胎移植中胚胎发育、妊娠结局以及子代出生缺陷的影响。
方法: 根据胚胎体外培养的氧浓度，将2013至2015年在浙江大学医学院附属妇产科医院行体外受精-胚胎移植的患者随机分为低氧浓度组（ n=2036，培养体系含氧气体积分数为5%）和高氧浓度组（ n=4617，培养体系含氧气体积分数为20%）。比较两组间受精率、优质胚胎率、临床妊娠率、异位妊娠率、流产率以及子代出生缺陷率等结果指标。
结果: 低氧浓度组优质胚胎率高于高氧浓度组（ P < 0.05），但两组间受精率、临床妊娠率、异位妊娠率、流产率和子代出生缺陷的发生率差异均无统计学意义（均 P＞0.05）。
结论: 体外受精-胚胎移植采用低氧环境培养可能有助于提高胚胎发育潜能，但对妊娠结局和子代出生缺陷的影响不明显。