Pharmacokinetic Comparison of Seven Major Bio-Active Components in Normal and Blood Stasis Rats after Oral Administration of Herb Pair Danggui-Honghua by UPLC-TQ/MS

Molecules. 2017 Oct 17;22(10):1746. doi: 10.3390/molecules22101746.

Abstract

The compatibility between Danggui (Angelicae Sinensis Radix) and Honghua (Carthami Flos) is a known herb pair, which could activate blood circulation and dissipate blood stasis effects. In this paper, we quantified seven main bio-active components (hydroxysafflor yellow A, caffeic acid, p-coumaric acid, kaempferol-3-O-rutinoside, ferulic acid, 3-n-butylphthalide, and ligustilide) in plasma samples in vivo by UPLC-TQ/MS method and investigatedwhether the pharmacokinetic (PK) behaviors of the seven components could be altered in blood stasis rats after oral administration of the Gui-Hong extracts. It was found that the Cmax and AUC0-t of these components in blood stasis rats had increasing tendency compared with normal rats. Most components in model and normal rats had significant difference in some pharmacokinetic parameters, which indicated that the metabolism enzymes and transporters involved in the metabolism and disposition of these bio-active componentsmay bealtered in blood stasis rats. This study was the first report about the pharmacokinetic investigation between normal and blood stasis rats after oral administrationof Gui-Hong extracts, and these results are important and valuable for better clinical applications of Gui-Hong herb pair and relatedTCM formulae.

Keywords: Angelica sinensis; Carthamus tinctorius; Danggui; herb pair; honghua; pharmacokinetics.

MeSH terms

  • Administration, Oral
  • Animals
  • Biological Products / administration & dosage
  • Biological Products / chemistry*
  • Biological Products / pharmacokinetics*
  • Biomarkers
  • Carthamus tinctorius / chemistry
  • Chromatography, High Pressure Liquid
  • Drugs, Chinese Herbal / administration & dosage
  • Drugs, Chinese Herbal / chemistry*
  • Drugs, Chinese Herbal / pharmacokinetics*
  • Female
  • Hematologic Tests
  • Mass Spectrometry
  • Molecular Structure
  • Quality Control
  • Rats
  • Sensitivity and Specificity

Substances

  • Biological Products
  • Biomarkers
  • Drugs, Chinese Herbal
  • Honghua extract, Carthamus tinctorius