Effects of polysaccharides from the base of Flammulina Velutipes stipe on growth of murine RAW264.7, B16F10 and L929 cells

Gui-Tang Chen; Ya-Xin Fu; Wen-Jian Yang; Qiu-Hui Hu; Li-Yan Zhao

doi:10.1016/j.ijbiomac.2017.10.090

Effects of polysaccharides from the base of Flammulina Velutipes stipe on growth of murine RAW264.7, B16F10 and L929 cells

Int J Biol Macromol. 2018 Feb;107(Pt B):2150-2156. doi: 10.1016/j.ijbiomac.2017.10.090. Epub 2017 Oct 16.

Authors

Gui-Tang Chen¹, Ya-Xin Fu², Wen-Jian Yang³, Qiu-Hui Hu⁴, Li-Yan Zhao⁵

Affiliations

¹ Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
² College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
³ College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China.
⁴ College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China; College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China.
⁵ College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China. Electronic address: zhlychen@njau.edu.cn.

PMID: 29042281
DOI: 10.1016/j.ijbiomac.2017.10.090

Abstract

A polysaccharide (FVSP) was isolated from the base of Flammuliana Velutipes stipe, and FVSP was further purified by DEAE-cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to obtain three fractions named FVSP-1, FVSP-2 and FVSP-3. Then their activation of macrophage cell RAW 264.7 and anti-proliferative effects to the murine melanoma B16F10 and fibroblasts L929 cells were evaluated by using the cell model experiments. The results indicated that the polysaccharide fractions could increase the proliferation and phagocytic activity of macrophage significantly and play an inhibited effect on the cancer cells. Moreover, the anti-proliferative activities of FVSPs increased with the participation of the antitumor factors induced from macrophage by polysaccharides fractions. Taken together, these results indicated that three polysaccharides fractions from the base of F. Velutipes stipe may be useful as potent antitumor agents for the prevention of tumorigenesis.

Keywords: Antitumor activity; B16F10; Flammulina velutipes; L929; Polysaccharides; RAW264.7.

MeSH terms

Animals
Cell Proliferation / drug effects
Coculture Techniques
Flammulina / chemistry*
Macrophages / cytology*
Macrophages / drug effects
Macrophages / metabolism
Melanoma, Experimental / pathology*
Mice
Neutral Red / metabolism
Phagocytosis / drug effects
Polysaccharides / pharmacology*
RAW 264.7 Cells
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet

Substances

Polysaccharides
Neutral Red