Imaging of DNA Ultrafine Bridges in Budding Yeast

Methods Mol Biol. 2018:1672:483-493. doi: 10.1007/978-1-4939-7306-4_32.

Abstract

DNA ultrafine bridges (UFBs) are a type of chromatin-free DNA bridges that connect sister chromatids in anaphase and pose a threat to genome stability. However, little is known about the origin of these structures, and how they are sensed and resolved by the cell. In this chapter, we review tools and methods for studying UFBs by fluorescence microscopy including chemical and genetic approaches to induce UFBs in the model organism Saccharomyces cerevisiae.

Keywords: Anaphase bridges; Fluorescence microscopy; UFBs; Ultrafine bridges; Yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase / genetics
  • Chromatids* / genetics
  • Chromatin* / genetics
  • DNA*
  • Genes, Fungal
  • Genomic Instability
  • Microscopy, Fluorescence
  • Molecular Imaging*
  • Mutation
  • Saccharomycetales / genetics*
  • Time-Lapse Imaging

Substances

  • Chromatin
  • DNA