Labeling the ER for Light and Fluorescence Microscopy

doi:10.1007/978-1-4939-7389-7_1

. 2018:1691:1-14.

doi: 10.1007/978-1-4939-7389-7_1.

Labeling the ER for Light and Fluorescence Microscopy

Chris Hawes¹, Pengwei Wang², Verena Kriechbaumer³

Affiliations

¹ Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK. chawes@brookes.ac.uk.
² Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, PR China.
³ Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK.

PMID: 29043665
DOI: 10.1007/978-1-4939-7389-7_1

Labeling the ER for Light and Fluorescence Microscopy

Chris Hawes et al. Methods Mol Biol. 2018.

. 2018:1691:1-14.

doi: 10.1007/978-1-4939-7389-7_1.

Authors

Chris Hawes¹, Pengwei Wang², Verena Kriechbaumer³

Affiliations

¹ Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK. chawes@brookes.ac.uk.
² Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, PR China.
³ Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK.

PMID: 29043665
DOI: 10.1007/978-1-4939-7389-7_1

Abstract

The ER is a highly dynamic network of tubules and membrane sheets. Hence imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labeling the native ER using fluorescent proteins and lipid dyes as well as methods for immunolabeling on plant tissue.

Keywords: Endoplasmic reticulum; Fluorescent dyes; Fluorescent protein; Immunofluorescence; Labeling; Stable expression; Transient expression.

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Labeling the ER for Light and Fluorescence Microscopy

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Labeling the ER for Light and Fluorescence Microscopy

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