A New Subspecies of Oxytricha Granulifera (Hypotrichia: Oxytrichidae) From Mexico, With Notes on Its Morphogenesis and Phylogenetic Position

J Eukaryot Microbiol. 2018 May;65(3):357-371. doi: 10.1111/jeu.12479. Epub 2017 Nov 10.

Abstract

The genus Oxytricha Bory de Saint-Vincent in Lamouroux, Bory de Saint-Vincent and Deslongchamps, 1824 comprises about 38 species distributed worldwide and has been considered to be a nonmonophyletic group. Based on living observations, protargol preparations, and a small subunit ribosomal RNA (SSU rRNA) gene sequence, we describe a new subspecies Oxytricha granulifera chiapasensis n. subsp. This new taxon is morphologically characterized by undulating membranes basically in a Stylonychia-pattern, six dorsal kineties, size in vivo ca. 60-120 × 20-40 μm, 21-30 right and 21-31 left marginal cirri, 22-29 adoral membranelles, and spherical cortical granules arranged in longitudinal rows on the dorsal side. In terms of the SSU rRNA gene sequence, the new subspecies differs from populations of O. granulifera from GENBANK by 7-35 nucleotides. Phylogenetic analyses showed that Oxytricha granulifera gene sequences were nested into three groups, with the new subspecies included in one of them. Oxytricha granulifera chiapasensis n. subsp. is different from Oxytricha granulifera granulifera Foissner and Adam, 1983 and Oxytricha granulifera quadricirrata Blatterer and Foissner, 1988 based on: (i) undulating membranes in Stylonychia-pattern, (ii) formation of a sixth dorsal kinety during morphogenesis, (iii) the adoral membranelles number, and (iv) inhabiting freshwater habitats.

Keywords: Oxytricha granulifera chiapasensis; Cell division; Chiapas; SSU rRNA; dorsal kineties; undulating membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Protozoan / genetics
  • DNA, Ribosomal / genetics
  • Ecosystem
  • Lakes / parasitology*
  • Mexico
  • Oxytricha / classification*
  • Oxytricha / genetics
  • Oxytricha / isolation & purification
  • Phylogeny
  • Ribosome Subunits, Small / genetics

Substances

  • DNA, Protozoan
  • DNA, Ribosomal

Associated data

  • GENBANK/FJ422988
  • GENBANK/DQ662848
  • GENBANK/FJ790209
  • GENBANK/DQ393786
  • GENBANK/KX889988
  • GENBANK/KU715983
  • GENBANK/X53486
  • GENBANK/KJ081199
  • GENBANK/AF164122
  • GENBANK/AM412772
  • GENBANK/AM412768
  • GENBANK/AM412769
  • GENBANK/AM412771
  • GENBANK/AM412770
  • GENBANK/JX899421
  • GENBANK/KJ000536
  • GENBANK/AF508766