Bladder regeneration in a canine model using a bladder acellular matrix loaded with a collagen-binding bFGF

Biomater Sci. 2017 Nov 21;5(12):2427-2436. doi: 10.1039/c7bm00806f.

Abstract

Bladder reconstruction remains challenging for urological surgery due to lack of suitable regenerative scaffolds. In a previous study, we had used a collagen-binding basic fibroblast growth factor (CBD-bFGF) to bind bFGF to the collagen scaffold, which could promote bladder regeneration in rats. However, the limited graft size in rodent models cannot provide enough evidence to demonstrate the repair capabilities of this method for severely damaged bladders in humans or large animals. In this study, the CBD-bFGF was used to activate a bladder acellular matrix (BAM) scaffold, and the CBD-bFGF/BAM functional scaffold was assessed in a canine model with a large segment defect (half of the entire bladder was resected). The results demonstrated that the functional biomaterials could promote bladder smooth muscle, vascular, and nerve regeneration and improve the function of neobladders. Thus, the CBD-bFGF/BAM functional scaffold may be a promising biomaterial for bladder reconstruction.

MeSH terms

  • Animals
  • Collagen / chemistry
  • Collagen / metabolism
  • Disease Models, Animal
  • Dogs
  • Fibroblast Growth Factor 2 / chemistry*
  • Fibroblast Growth Factor 2 / metabolism
  • Humans
  • Protein Binding
  • Regeneration*
  • Regenerative Medicine / methods
  • Tissue Scaffolds*
  • Urinary Bladder / drug effects
  • Urinary Bladder / growth & development*
  • Urinary Bladder / physiopathology

Substances

  • Fibroblast Growth Factor 2
  • Collagen