DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25

S L Graziano; G E Mark 3rd; C Murray; D L Mann; G D Ehrlich; B J Poiesz; A Weston

DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25

Oncogene Res. 1988;3(1):99-103.

Authors

S L Graziano¹, G E Mark 3rd, C Murray, D L Mann, G D Ehrlich, B J Poiesz, A Weston

Affiliation

¹ Department of Medicine, SUNY-Health Science Center, Syracuse 13210.

PMID: 2905034

Abstract

We report the identification and characterization of two restriction fragment polymorphisms (RFLPs) associated with the human c-raf-1 protooncogene located on chromosome 3 (3p25). EcoRI digestion produces an RFLP derived from the 5'flanking sequences with a heterozygosity of 28%, and TaqI produces an RFLP representing the 3' flanking sequences with a heterozygosity of 25%. The finding of RFLPs associated with a potential oncogene may provide a method of analysis for determining genetic susceptibilities to certain cancers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Chromosomes, Human, Pair 3*
Humans
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-raf

Substances

Proto-Oncogene Proteins
Proto-Oncogene Proteins c-raf