Genotoxic evaluation of newly synthesized iminothiazolidinones

Toxicol Ind Health. 2017 Nov;33(11):811-820. doi: 10.1177/0748233717730492. Epub 2017 Oct 19.


The current study was designed to assess the potential toxicological effects of newly synthesized iminothiazolidinones by employing Ames Salmonella, Escherichia coli WP2, Zea mays seed germination, and random amplified polymorphic DNA (RAPD) assay systems. The bacterial tester strains S. typhimurium TA1535, TA1537, TA1538, TA98, TA100, and E. coli WP2 uvrA were chosen to test the direct gene mutation inducing capabilities of the test materials in prokaryotic systems and Z. mays seeds for determination of potential toxicological effects in eukaryotic systems. OPA-3 and OPA-6 primers were used in the RAPD analysis to determine genotoxic activities on the eukaryotic genomes. According to the results, none of the test materials showed significant mutagenic activity on the bacterial tester strains at the chosen concentrations. Additionally, none of the tested compounds showed inhibition of the germination of Z. mays seeds. In contrast, the RAPD analysis results were inconsistent with the bacterial reversion assays and the seed germination assay results. All test materials significantly changed the RAPD profiles for OPA-3; however, only compound 5 showed a significant change for OPA-6 when compared with the control groups. In conclusion, the newly synthesized iminothiazolidinone derivatives (C1-C5) were determined as potentially genotoxic compounds and they should be checked with multiple toxicology test systems before further studies to determine their actual use.

Keywords: Ames Salmonella assay; E. coli WP2 assay; Iminothiazolidinones; RAPD; Z. mays seed germination.

MeSH terms

  • Anti-Bacterial Agents / adverse effects*
  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry
  • Antitubercular Agents / adverse effects
  • Antitubercular Agents / chemical synthesis
  • Antitubercular Agents / chemistry
  • Drug Design*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Germination / drug effects
  • Microbial Viability / drug effects
  • Molecular Structure
  • Mutagenesis / drug effects*
  • Mutagenicity Tests
  • Pesticides / chemical synthesis
  • Pesticides / chemistry
  • Pesticides / toxicity*
  • Random Amplified Polymorphic DNA Technique
  • Salmonella / drug effects
  • Salmonella / growth & development
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / growth & development
  • Seeds / drug effects
  • Seeds / growth & development
  • Thiazoles / chemical synthesis
  • Thiazoles / chemistry
  • Thiazoles / toxicity*
  • Zea mays / drug effects
  • Zea mays / growth & development


  • Anti-Bacterial Agents
  • Antitubercular Agents
  • Pesticides
  • Thiazoles