A new locus (NIA 1) in Arabidopsis thaliana encoding nitrate reductase

EMBO J. 1988 Nov;7(11):3309-14.

Abstract

We have isolated two nitrate reductase genes and their corresponding cDNAs from Arabidopsis thaliana. Sequences of the two cDNAs, when compared to a sequence of a barley cDNA clone, confirm their identity as nitrate reductase clones and show that they are closely related. The two genes have been mapped using restriction fragment length polymorphisms; gNR2 is close to the previously identified chl-3 locus and is probably identical to it, while gNR1 maps to a new locus (NIA1) on chromosome 1, near gl-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Centrifugation, Density Gradient
  • Cloning, Molecular
  • DNA / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Sequence Data
  • Nitrate Reductase
  • Nitrate Reductases / genetics*
  • Plants / enzymology
  • Plants / genetics*
  • Polymorphism, Restriction Fragment Length
  • Precipitin Tests
  • Restriction Mapping

Substances

  • DNA
  • Nitrate Reductases
  • Nitrate Reductase