A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection

Ning Wang; Di Zhang; Xinyu Deng; Ying Sun; Xinghua Wang; Pinyi Ma; Daqian Song

doi:10.1016/j.saa.2017.10.039

A novel surface plasmon resonance biosensor based on the PDA-AgNPs-PDA-Au film sensing platform for horse IgG detection

Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15:191:290-295. doi: 10.1016/j.saa.2017.10.039. Epub 2017 Oct 12.

Authors

Ning Wang¹, Di Zhang², Xinyu Deng², Ying Sun², Xinghua Wang², Pinyi Ma³, Daqian Song⁴

Affiliations

¹ Department of Medical Science & Education, Jilin Province People's Hospital, Changchun 130021, PR China.
² College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
³ College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: mapinyi@jlu.edu.cn.
⁴ College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: songdq@jlu.edu.cn.

PMID: 29054067
DOI: 10.1016/j.saa.2017.10.039

Abstract

Herein we report a novel polydopamine-silver nanoparticle-polydopamine-gold (PDA-AgNPs-PDA-Au) film based surface plasmon resonance (SPR) biosensor for horse IgG detection. The PDA-AgNPs-PDA-Au film sensing platform was built on Au-film via layer-by-layer self-assembly. Ag ion was reduced in situ to AgNPs in presence of PDA. The top PDA layer can prevent AgNPs from being oxidized and connect with antibody via Schiff alkali reaction directly. The morphology and thickness of the modified gold film were characterized using scanning electron microscope and Talystep. Experimental results show that the PDA-AgNPs-PDA-Au film sensing platform is stable, regenerative and sensitive for horse IgG detection. The detection limit of horse IgG obtained with the present biosensor is 0.625μgmL^-1, which is 2-fold and 4-fold lower than that obtained with biosensor based on PDA modified Au film and conventional biosensor based on MPA, respectively. Furthermore, when challenged to real serum samples, our sensor exhibited excellent specificity to horse IgG, suggesting its potential for industrial application.

Keywords: Ag nanoparticles; Horse IgG; Polydopamine; Surface plasmon resonance.

MeSH terms

Animals
Biosensing Techniques / methods*
Horses
Humans
Immunoglobulin G / analysis*
Indoles / chemistry*
Kinetics
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Polymers / chemistry*
Protein Stability
Silver / chemistry*
Surface Plasmon Resonance

Substances

Immunoglobulin G
Indoles
Polymers
polydopamine
Silver