Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger

BMC Res Notes. 2017 Oct 23;10(1):505. doi: 10.1186/s13104-017-2832-1.

Abstract

Objective: We conducted a parallel evaluation of the diagnostic accuracy of VIKIA® Rota-Adeno, a rapid diagnostic test (RDT) and Premier™ Rotaclone® an enzyme immunoassay (EIA) using reverse transcription polymerase chain reaction (RT-PCR) as the reference standard. The study was part of a rotavirus surveillance project in Niger.

Results: The sensitivity of both tests was 80.7%. After exclusion of one indeterminate result by visual reading, the specificity of the Premier™ Rotaclone® was 100% by visual or optical density readings and that of VIKIA® Rota-Adeno test was 95.5%. Inter-reader agreement was excellent for both tests (kappa = 1). Our results showed almost similar performance of the EIA and RDT when compared to RT-PCR. Hence, the VIKIA® Rota-Adeno could be a good alternative for use in peripheral health centres where laboratory capacity is limited.

Keywords: RT-PCR; Rotaclone; Rotavirus; Sensitivity; Specificity; VIKIA.

Publication types

  • Evaluation Study

MeSH terms

  • Child, Preschool
  • Chromatography, Affinity / standards*
  • Feces / virology*
  • Humans
  • Immunoenzyme Techniques / standards*
  • Infant
  • Niger
  • Reagent Kits, Diagnostic / standards*
  • Reverse Transcriptase Polymerase Chain Reaction / standards*
  • Rotavirus Infections / diagnosis*
  • Rotavirus*
  • Sensitivity and Specificity

Substances

  • Reagent Kits, Diagnostic