Control of plant cell fate transitions by transcriptional and hormonal signals

Elife. 2017 Oct 23;6:e30135. doi: 10.7554/eLife.30135.

Abstract

Plant meristems carry pools of continuously active stem cells, whose activity is controlled by developmental and environmental signals. After stem cell division, daughter cells that exit the stem cell domain acquire transit amplifying cell identity before they are incorporated into organs and differentiate. In this study, we used an integrated approach to elucidate the role of HECATE (HEC) genes in regulating developmental trajectories of shoot stem cells in Arabidopsis thaliana. Our work reveals that HEC function stabilizes cell fate in distinct zones of the shoot meristem thereby controlling the spatio-temporal dynamics of stem cell differentiation. Importantly, this activity is concomitant with the local modulation of cellular responses to cytokinin and auxin, two key phytohormones regulating cell behaviour. Mechanistically, we show that HEC factors transcriptionally control and physically interact with MONOPTEROS (MP), a key regulator of auxin signalling, and modulate the autocatalytic stabilization of auxin signalling output.

Keywords: A. thaliana; HECATE1; auxin; cell fate; cytokinin; developmental biology; plant biology; shoot meristem; stem cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Arabidopsis / physiology*
  • Cell Differentiation / drug effects*
  • Gene Expression Regulation, Plant / drug effects*
  • Genes, Plant
  • Plant Cells / drug effects
  • Plant Cells / physiology*
  • Plant Growth Regulators / metabolism*
  • Plant Shoots / physiology
  • Stem Cells / drug effects
  • Stem Cells / physiology*
  • Transcription, Genetic

Substances

  • Plant Growth Regulators