Activation of a bradykinin receptor in peripheral nerve and spinal cord in the neonatal rat in vitro

Br J Pharmacol. 1988 Dec;95(4):1008-10. doi: 10.1111/j.1476-5381.1988.tb11732.x.


In an in vitro preparation of the neonatal rat spinal cord with attached tail, administration of bradykinin (Bk) to the spinal cord or to the tail produced depolarization of a ventral root (L3-L5). The effect of Bk at each site was selectively and reversibly antagonized by D-Arg [Hyp2, Thi5,8 D-Phe7]-Bk but could not be mimicked or antagonized by the B1-receptor ligands [des-Arg9]-Bk or Leu8[des-Arg9]-Bk, respectively. Peripherally evoked noxious responses produced by capsaicin or heat, were unaffected by either antagonist administered to the spinal cord. These data suggest that Bk-evoked responses in the spinal cord and at peripheral nociceptors were mediated via a receptor which by definition is of the B2-type. Additionally Bk is unlikely to be a physiological mediator of acute nociception in the spinal cord.

MeSH terms

  • Animals
  • Animals, Newborn
  • Bradykinin / analogs & derivatives
  • Bradykinin / pharmacology
  • Bradykinin / physiology*
  • In Vitro Techniques
  • Membrane Potentials / drug effects
  • Peripheral Nerves / drug effects
  • Peripheral Nerves / physiology*
  • Rats
  • Receptors, Bradykinin
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / physiology*
  • Spinal Cord / drug effects
  • Spinal Cord / physiology*


  • Receptors, Bradykinin
  • Receptors, Neurotransmitter
  • HOE k86-4321
  • bradykinin, des-Arg(9)-
  • bradykinin, Leu(8)-des-Arg(9)-
  • Bradykinin