Candidate recommendations for protein electrophoresis reporting from the Canadian Society of Clinical Chemists Monoclonal Gammopathy Working Group

Ronald A Booth; Christopher R McCudden; Cynthia M Balion; Ivan M Blasutig; Ihssan Bouhtiauy; Karina Rodriguez-Capote; Peter Catomeris; Pak Cheung Chan; Yu Chen; Christine Collier; Kristin Hauff; Jawahar Kalra; Dailin Li; Dan C Lin; Amy H Lou; Qing H Meng; Tracy Morrison; Maria D Pasic; Mabood Qureshi; Ed Randell; Kun-Young Sohn; Vinita Thakur; Dylan Thomas; Andrea Thoni; Cheryl Tomalty; Liju Yang; Mohebullah Zamkanei

doi:10.1016/j.clinbiochem.2017.10.013

Candidate recommendations for protein electrophoresis reporting from the Canadian Society of Clinical Chemists Monoclonal Gammopathy Working Group

Clin Biochem. 2018 Jan:51:10-20. doi: 10.1016/j.clinbiochem.2017.10.013. Epub 2017 Oct 20.

Authors

Ronald A Booth¹, Christopher R McCudden², Cynthia M Balion³, Ivan M Blasutig⁴, Ihssan Bouhtiauy⁵, Karina Rodriguez-Capote⁶, Peter Catomeris⁷, Pak Cheung Chan⁸, Yu Chen⁹, Christine Collier¹⁰, Kristin Hauff¹¹, Jawahar Kalra¹², Dailin Li¹³, Dan C Lin², Amy H Lou¹⁴, Qing H Meng¹⁵, Tracy Morrison¹⁶, Maria D Pasic¹⁷, Mabood Qureshi¹⁸, Ed Randell¹⁹, Kun-Young Sohn²⁰, Vinita Thakur²¹, Dylan Thomas²², Andrea Thoni²³, Cheryl Tomalty¹¹, Liju Yang²⁴, Mohebullah Zamkanei²⁵

Affiliations

¹ Division of Biochemistry, The Ottawa Hospital and Department of Pathology & Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada. Electronic address: rbooth@toh.ca.
² Division of Biochemistry, The Ottawa Hospital and Department of Pathology & Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada.
³ Hamilton Regional Laboratory Medicine Program and Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
⁴ Division of Biochemistry, Children's Hospital of Eastern Ontario, Ottawa, ON, Canada.
⁵ Centre hospitalier Universitaire Georges-L-Dumont, Moncton, New Brunswick, Canada.
⁶ DynaLIFE Medical Labs & Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
⁷ Dynacare, Brampton, ON, Canada.
⁸ Sunnybrook Health Sciences Center & University of Toronto, ON, Canada.
⁹ Department of Laboratory Medicine, Dr. Everett Chalmers Regional Hospital & Department of Pathology, Dalhousie University, Fredericton, NB, Canada.
¹⁰ Kingston General Hospital, Department of Pathology & Molecular Medicine, Queen's University, Kingston, ON, Canada.
¹¹ LifeLabs, Burnaby, BC, Canada.
¹² Department of Pathology and Laboratory medicine, University of Saskatchewan and Saskatoon health region, Saskatoon, SK, Canada.
¹³ Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
¹⁴ Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority- Central Zone, Dalhousie University, Halifax, NS, Canada.
¹⁵ Division of Pathology and Lab Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
¹⁶ LifeLabs, Toronto, ON, Canada.
¹⁷ Department of Laboratory Medicine, St. Joseph's Health Centre and St. Michael's Hospital, Toronto & Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
¹⁸ Royal University Hospital and Department of Pathology and Laboratory Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
¹⁹ Laboratory Medicine, Eastern Health Authority & Faculty of Medicine, Memorial University, St. John's, NL, Canada.
²⁰ Trillium Health Partners, Mississauga, Ontario & University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, ON, Canada.
²¹ Department of Lab Medicine, Batra Hospital,New Delhi, India.
²² DynaLIFE Medical Labs & Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
²³ Nova Scotia Health Authority, Department of Pathology and Laboratory Medicine, Halifax, NS, Canada.
²⁴ Department of Pathology and Laboratory Medicine, London Health Sciences Centre, London, ON, Canada.
²⁵ Grand River Hospital, Kitchener, ON, Canada.

PMID: 29061378
DOI: 10.1016/j.clinbiochem.2017.10.013

Abstract

Protein electrophoresis is commonly used as an aid in the diagnosis of monoclonal gammopathies and is performed in many laboratories in Canada and throughout the world. However, unlike many other diagnostic tests, there is limited guidance for standardization and neither guidance nor specific recommendations for clinical reporting of serum (SPE) or urine (UPE) protein electrophoresis and immunotyping available in the literature. Therefore, a Canadian effort was undertaken to recommend standards that cover all aspects of clinical reporting with an ultimate goal towards reporting standardization. The Canadian Society of Clinical Chemists (CSCC) Monoclonal Gammopathy Interest Group (MGIG), which is composed of CSCC members with an interest in protein electrophoresis, has formed a Monoclonal Gammopathy Working Group (MGWG) to take initial steps towards standardization of SPE, UPE and immunotyping. Candidate standardization recommendations were developed, discussed and voted upon by the MGWG. Candidate recommendations that achieved 90% agreement are presented as consensus recommendations. Recommendations that did not achieve 90% consensus remain candidate recommendations and are presented with accompanying MGWG discussion. Eleven consensus recommendations along with candidate recommendations for nomenclature, protein fraction reporting, test utilization, interference handling and interpretive reporting options are presented.

Keywords: Consensus; Monoclonal gammopathy; Monoclonal protein; Protein electrophoresis; Recommendations; Reporting.

MeSH terms

Blood Protein Electrophoresis / methods*
Canada
Guidelines as Topic*
Humans
Paraproteinemias / blood*
Societies, Medical*