Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma

Bull Exp Biol Med. 2017 Oct;163(6):777-779. doi: 10.1007/s10517-017-3901-0. Epub 2017 Oct 24.

Abstract

We studied the effects of a concentrate of metabolites of Trichoderma harzianum Rifai F-180, an active producer of L-lysine-α-oxidase, and homogenous enzyme on a highly virulent bacteria Erwinia amylovora. The producer of antitumor and antiviral Trichoderma enzyme L-lysine-α-oxidase was cultured on a processing system of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). Activity of L-lysine-α-oxidase in the prepared concentrate of metabolic products of the producer was 5.4 U/ml, and activity of the homogenous enzyme was 50 U/ml. Antibacterial activity of the enzyme was shown in our experiments.

Keywords: Erwinia amylovora; Trichoderma culture Fluid; antitumor enzyme L-lysine-α-oxidase.

MeSH terms

  • Amino Acid Oxidoreductases / isolation & purification
  • Amino Acid Oxidoreductases / pharmacology*
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Culture Media / chemistry
  • Erwinia amylovora / drug effects
  • Erwinia amylovora / growth & development
  • Fermentation
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / pharmacology*
  • Microbial Sensitivity Tests
  • Trichoderma / chemistry*
  • Trichoderma / enzymology

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Fungal Proteins
  • Amino Acid Oxidoreductases
  • L-lysine oxidase