MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: A prospective study

Pierre Thouvenot; Guillaume Vales; Hélène Bracq-Dieye; Nathalie Tessaud-Rita; Mylène M Maury; Alexandra Moura; Marc Lecuit; Alexandre Leclercq

doi:10.1016/j.mimet.2017.10.009

MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: A prospective study

J Microbiol Methods. 2018 Jan:144:29-32. doi: 10.1016/j.mimet.2017.10.009. Epub 2017 Oct 21.

Authors

Pierre Thouvenot¹, Guillaume Vales¹, Hélène Bracq-Dieye¹, Nathalie Tessaud-Rita¹, Mylène M Maury¹, Alexandra Moura¹, Marc Lecuit², Alexandre Leclercq³

Affiliations

¹ Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
² Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France; Inserm U1117, Paris, France; Paris Descartes University, Necker-Enfants Malades University Hospital, Department of Infectious Diseases and Tropical Medicine, Institut Imagine, APHP, Paris, France. Electronic address: marc.lecuit@pasteur.fr.
³ Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France. Electronic address: alexandre.leclercq@pasteur.fr.

PMID: 29066314
DOI: 10.1016/j.mimet.2017.10.009

Abstract

This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification of L. monocytogenes, L. innocua, L. ivanovii, L. fleischmannii, L. grayi, L. seeligeri, L. weihenstephanensis and L. welshimeri, as confirmed by whole genome sequence analyses.

Keywords: Food; Listeria identification; MALDI-TOF; Mass spectrometry; Public health surveillance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Computational Biology / methods
Food Microbiology
Listeria / classification*
Listeria / genetics
Listeria / isolation & purification*
Prospective Studies
Public Health Surveillance
Sequence Analysis, DNA / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Whole Genome Sequencing