Cell cycle dependent distribution of the proliferation-associated Ki-67 antigen in human embryonic lung cells

Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(1):25-33. doi: 10.1007/BF02889998.


The cell cycle-dependent distribution of the proliferation-associated Ki-67 antigen has been evaluated immunocytochemically in L-132 human fetal lung cells. The cells were synchronized and cell cycle phases were determined: G1 = 6.7 h, S = 5.4 h, G2 = 8.5 h and mitosis = 1.3 h. The Ki-67 patterns were strictly correlated with the cell cycle phases. In late G1-phase, Ki-67 antigen was present only in the perinucleolar region. In the S-phase, Ki-67 staining was found homogeneously in the karyoplasm and in the perinucleolar region. G2-phase cells contained a finely granular Ki-67 staining in the karyoplasm with Ki-67-positive specks and perinucleolar staining. In early mitotic cells (pro- and metaphase) an intense perichromosomal Ki-67 staining was observed in addition to a homogeneously stained karyoplasm in prophase, and cytoplasm in metaphase. During ana- and telophase the Ki-67 antigen disappeared rapidly. In resting cells there was no Ki-67 staining.

MeSH terms

  • Antigens / immunology*
  • Cell Cycle*
  • Cell Division
  • Cell Line
  • Embryo, Mammalian
  • Humans
  • Immunohistochemistry
  • Lung


  • Antigens