Identification and functional characterization of muscle satellite cells in Drosophila

Elife. 2017 Oct 26;6:e30107. doi: 10.7554/eLife.30107.

Abstract

Work on genetic model systems such as Drosophila and mouse has shown that the fundamental mechanisms of myogenesis are remarkably similar in vertebrates and invertebrates. Strikingly, however, satellite cells, the adult muscle stem cells that are essential for the regeneration of damaged muscles in vertebrates, have not been reported in invertebrates. In this study, we show that lineal descendants of muscle stem cells are present in adult muscle of Drosophila as small, unfused cells observed at the surface and in close proximity to the mature muscle fibers. Normally quiescent, following muscle fiber injury, we show that these cells express Zfh1 and engage in Notch-Delta-dependent proliferative activity and generate lineal descendant populations, which fuse with the injured muscle fiber. In view of strikingly similar morphological and functional features, we consider these novel cells to be the Drosophila equivalent of vertebrate muscle satellite cells.

Keywords: D. melanogaster; Zfh-1; developmental biology; muscle repair; stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation
  • Drosophila / physiology*
  • Drosophila Proteins / metabolism
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Membrane Proteins / metabolism
  • Muscle Development*
  • Muscle Fibers, Skeletal / cytology*
  • Muscles / injuries
  • Repressor Proteins / metabolism
  • Satellite Cells, Skeletal Muscle / physiology*

Substances

  • Drosophila Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Repressor Proteins
  • delta protein
  • zfh1 protein, Drosophila

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.