J Allergy Clin Immunol. 2018 Apr;141(4):1202-1207. doi: 10.1016/j.jaci.2017.08.034. Epub 2017 Oct 23.


MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNA research in allergy is expanding because miRNAs are crucial regulators of gene expression and promising candidates for biomarker development. MiRNA mimics and miRNA inhibitors currently in preclinical development have shown promise as novel therapeutic agents. Multiple technological platforms have been developed for miRNA isolation, miRNA quantitation, miRNA profiling, miRNA target detection, and modulating miRNA levels in vitro and in vivo. Here we will review the major technological platforms with consideration given for the advantages and disadvantages of each platform.

Keywords: Allergy; CRISPR; CRISPR/Cas9; antagomiRs; antimiRs; gene silencing; in situ hybridization; miRNA; miRNA expression; miRNA inhibitors; miRNA isolation; miRNA mimics; miRNA profiling; miRNA quantitation; miRNA targets; microRNA; microRNA methods; microarray; qPCR; stem-loop.

Publication types

  • Review

MeSH terms

  • Gene Expression Regulation
  • Genetic Markers
  • Humans
  • Hypersensitivity / drug therapy
  • Hypersensitivity / genetics*
  • Hypersensitivity / metabolism
  • In Vitro Techniques
  • MicroRNAs* / genetics
  • MicroRNAs* / isolation & purification
  • MicroRNAs* / metabolism
  • MicroRNAs* / therapeutic use


  • Genetic Markers
  • MicroRNAs