Temperature dependence of unwinding forces between complementary oligonucleotides

Fedor N Dultsev; Nina N Kurus

doi:10.1016/j.mimet.2017.10.011

Temperature dependence of unwinding forces between complementary oligonucleotides

J Microbiol Methods. 2017 Dec:143:94-97. doi: 10.1016/j.mimet.2017.10.011. Epub 2017 Oct 26.

Authors

Fedor N Dultsev¹, Nina N Kurus²

Affiliations

¹ Institute of Semiconductor Physics, SB RAS, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk 630090, Russia. Electronic address: fdultsev@isp.nsc.ru.
² Institute of Semiconductor Physics, SB RAS, Novosibirsk 630090, Russia.

PMID: 29079297
DOI: 10.1016/j.mimet.2017.10.011

Abstract

Rupture Event Scanning (REVS) was used to study oligonucleotide unwinding under mechanical load. Oligonucleotide melting temperature was successfully estimated using this method. To estimate the enthalpy of reaction, we represented denaturation process as a unimolecular reaction. This gave us the possibility to recover the force profile from the experimental data obtained in force measurements at different scanning time (reaction time) for different temperatures.

Keywords: Bond rupture; DNA duplex; QCM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
Nucleic Acid Conformation / radiation effects*
Nucleic Acid Denaturation / radiation effects*
Oligonucleotides / metabolism*
Transition Temperature*

Substances

Oligonucleotides