Fluorescence-based cell-specific detection for laser-capture microdissection in human brain

Sci Rep. 2017 Oct 27;7(1):14213. doi: 10.1038/s41598-017-14484-9.


Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Brain / cytology*
  • Brain / metabolism
  • Gene Expression Profiling
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Laser Capture Microdissection / methods*
  • Male
  • Middle Aged
  • Young Adult