Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies

Angew Chem Int Ed Engl. 2018 Apr 9;57(16):4329-4337. doi: 10.1002/anie.201709201. Epub 2018 Feb 28.

Abstract

The discovery of the CRISPR-Cas9 endonuclease has enabled facile genome editing in living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind DNA in an RNA-guided fashion, and has additionally been explored as a tool for transcriptional modulation, epigenetic editing, and genome imaging. This Review highlights recent progress and challenges in the development of dCas9 for imaging genomic loci. The emergence and maturation of this technology offers the potential to answer mechanistic questions about chromosome dynamics and three-dimensional genome organization in vivo.

Keywords: 3D genome organization; CRISPR; genome editing; imaging; microscopy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • CRISPR-Associated Protein 9 / metabolism*
  • CRISPR-Cas Systems / genetics*
  • Genome / genetics*
  • Humans
  • Molecular Imaging / methods*

Substances

  • CRISPR-Associated Protein 9