SDA and IDA - Two aptamers to inhibit cancer cell adhesion

Biochimie. 2018 Feb:145:84-90. doi: 10.1016/j.biochi.2017.10.018. Epub 2017 Nov 2.

Abstract

Aptamers which bind to proteins involved in cell-cell interactions could have significant value to directly affect cancer cell adhesion or for directed cargo delivery. Here, I discuss two aptamers: aptamer SDA which binds to E- and P-selectin, and aptamer IDA which binds to α6β4 integrin. Both aptamers (SDA 91 nt and IDA 77 nt) bind their target proteins with dissociation constants in the 100-150 nM range and substantially inhibit special cellular adhesion, possibly a first and pivotal step in transendothelial migration during metastasis formation. The aptamers' half-lives in cell culture media are between two and six hours. IDA is internalized by integrin presenting cells within minutes thus possibly serving as vehicle for directed cargo delivery.

Keywords: Aptamers; Cell adhesion; DNA aptamers; Human integrin; Human selectin; Metastasis.

Publication types

  • Review

MeSH terms

  • Aptamers, Nucleotide* / chemical synthesis
  • Aptamers, Nucleotide* / chemistry
  • Aptamers, Nucleotide* / therapeutic use
  • Cell Adhesion / drug effects
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • E-Selectin / antagonists & inhibitors*
  • E-Selectin / metabolism
  • Humans
  • Integrin alpha6beta4 / antagonists & inhibitors*
  • Integrin alpha6beta4 / metabolism
  • Neoplasm Metastasis
  • Neoplasm Proteins / antagonists & inhibitors*
  • Neoplasm Proteins / metabolism
  • Neoplasms* / drug therapy
  • Neoplasms* / metabolism
  • Neoplasms* / pathology
  • P-Selectin / antagonists & inhibitors*
  • P-Selectin / metabolism

Substances

  • Aptamers, Nucleotide
  • E-Selectin
  • Integrin alpha6beta4
  • Neoplasm Proteins
  • P-Selectin