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. 2017 Oct;14(4):4659-4662.
doi: 10.3892/ol.2017.6823. Epub 2017 Aug 24.

Long non-coding RNA PTENP1 inhibits proliferation and migration of breast cancer cells via AKT and MAPK signaling pathways

Sheng Chen  1 , Ye Wang  2 , Jian-Hua Zhang  1 , Qi-Jun Xia  1 , Qiang Sun  1 , Zhen-Kai Li  1 , Jian-Guo Zhang  1 , Mao-Sheng Tang  1 , Mao-Sheng Dong  1
Affiliations

Long non-coding RNA PTENP1 inhibits proliferation and migration of breast cancer cells via AKT and MAPK signaling pathways

Sheng Chen et al. Oncol Lett. 2017 Oct.

Abstract

We aimed to investigate the influence of long non-coding RNA (lncRNA) PTEN pseudogene-1 (PTENP1) on the proliferation, migration and cycle of breast cancer cells and its mechanism. Lentiviral vectors expressing PTENP1 were synthesized and breast cancer cells MCF7 were transfected with LV003-GFP-PTENP1 and LV003-GFP, respectively. The proliferation capacities of breast cancer cells were detected using CCK-8 assay, and the migration capacities of breast cancer cells were detected using scratch assay; flow cytometry was used to detect the cell cycles and Western blot was used to detect the expression levels of cyclin A2, CDK2, p-p44/42 MAPK, t-p44/42 MAPK, p-p38 MAPK, t-p38 MAPK, p-AKT, t-AKT in AKT and MAPK pathways. The absorbance values (A450) of cells in experimental group at 48 and 72 h were 1.4±0.3 and 2.3±0.47, respectively, which were significantly lower than those in control group (3.2±0.39, 3.4±0.58) (P<0.05). The number of cell colonies in experimental group was (48±13), which was significantly lower than that in control group (159±16) (P<0.01). The cell migration rate in experimental group was 22.8±3.3%, which was significantly lower than that in control group 61.8±5.2% (P<0.01). Western blot detection showed that the expression levels of cyclin A2, CDK2, p-AKT, p-p44/42 MAPK and p-p38 MAPK in experimental group were significantly decreased compared with those in control group. LncRNA PTENP1 can inhibit the proliferation and migration of breast cancer cells via the AKT and MAPK signaling pathways.

Keywords: PTENP1; breast cancer; cell cycle; lncRNA; migration.

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Figures

Figure 1.
Figure 1.
The growth curve of breast cancer MCF7 cells.
Figure 2.
Figure 2.
Colony-forming assay of breast cancer MCF7 cells. (A) Control group; (B) experimental group; (C) compared with control group, colony number was significantly reduced in the experimental group (**P<0.01).
Figure 3.
Figure 3.
Influence of lncRNA PTENP1 on migration capacity of breast cancer MCF7 cells between control group and experimental group (**P<0.01).
Figure 4.
Figure 4.
Influence of lncRNA PTENP1 on cyclin of breast cancer MCF7 cells between control group and experimental group.
Figure 5.
Figure 5.
Influence of lncRNA PTENP1 on AKT and MAPK signaling pathways of breast cancer MCF7 cells between control group and experimental group.
See this image and copyright information in PMC

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