Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas

Int J Mol Sci. 2017 Oct 31;18(11):2286. doi: 10.3390/ijms18112286.


Mammalian zygote-mediated genome-engineering by Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas is currently used for the generation of genome-modified animals. Here, we report that a Campylobacter jejuni-derived orthologous CRISPR/Cas system recognizes a 5'-NNNVRYAC sequence as a protospacer-adjacent motif in mouse zygotes, and is applicable for efficient generation of knockout mice. Moreover, this novel CRISPR/Cas can be used for zygote-mediated knock-in at a unique locus, suggesting that this system could help to expand the feasibility of the zygote-mediated generation of genome-modified animals.

Keywords: CRISPR/Cas; Campylobacter jejuni; genome-modified mouse.

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Campylobacter jejuni / enzymology
  • Campylobacter jejuni / genetics*
  • Female
  • Gene Editing / methods*
  • HEK293 Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL