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Published Erratum
. 2017 Oct 31;8(5):e01643-17.
doi: 10.1128/mBio.01643-17.

Erratum for Darkoh et al., "Accessory Gene Regulator-1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile"

Charles Darkoh  1   2 , Chioma Odo  3 , Herbert L DuPont  3   2
Affiliations
Free PMC article
Published Erratum

Erratum for Darkoh et al., "Accessory Gene Regulator-1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile"

Charles Darkoh et al. mBio. .
Free PMC article
No abstract available

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Figures

FIG 4
FIG 4
Culture supernatant fluids collected from the agrB1D1 mutants are not cytotoxic to human foreskin fibroblast cells. Strain 630 and R20291 agrB1D1 and agrB2D2 mutants were cultured for 48 h in BHI medium anaerobically at 37°C. The culture supernatant fluids were collected, filter sterilized with a 0.45-µm filter, and examined for cytotoxicity with the Bartels Clostridium difficile cytotoxicity assay kit (Trinity Biotech, Jamestown, NY). The culture fluids were incubated with the fibroblast cells for 24 h and observed under a microscope for cytotoxic effects. Images were taken with an EVOS XL microscope (Advanced Microscopy Group) at ×20 magnification. Panels: A, a representative image of fibroblast cells cultured in growth medium only; B, wild-type 630; C, 630 agrB1D1 mutant; D, 630 complemented agrB1D1 mutant; E, wild-type R20291; F, R20291 agrB1D1 mutant; G, complemented R20291 agrB1D1 mutant; H, R20291 agrB2D2 mutant.
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