Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec;63(3-4):422-430.
doi: 10.1007/s12031-017-0993-y. Epub 2017 Oct 31.

Endogenous TRPV4 Expression of a Hybrid Neuronal Cell Line N18D3 and Its Utilization to Find a Novel Synthetic Ligand

Affiliations

Endogenous TRPV4 Expression of a Hybrid Neuronal Cell Line N18D3 and Its Utilization to Find a Novel Synthetic Ligand

Sungjae Yoo et al. J Mol Neurosci. .

Abstract

Primary sensory afferent neurons detect environmental and painful stimuli at their peripheral termini. A group of transient receptor potential ion channels (TRPs) are expressed in these neurons and constitute sensor molecules for the stimuli such as thermal, mechanical, and chemical insults. We examined whether a mouse sensory neuronal line, N18D3, shows the sensory TRP expressions and their functionality. In Ca2+ imaging and electrophysiology with these cells, putative TRPV4-mediated responses were observed. TRPV4-specific sensory modalities including sensitivity to a specific agonist, hypotonicity, or an elevated temperature were reproduced in N18D3 cells. Electrophysiological and pharmacological profiles conformed to those from native TRPV4 of primarily cultured neurons. The TRPV4 expression in N18D3 was also confirmed by RT-PCR and Western blot analyses. Thus, N18D3 cells may represent TRPV4-expressing sensory neurons. Further, using this cell lines, we discovered a novel synthetic TRPV4-specific agonist, MLV-0901. These results suggest that N18D3 is a reliable cell line for functional and pharmacological TRPV4 assays. The chemical information from the novel agonist will contribute to TRPV4-targeting drug design.

Keywords: MLV-0901; N18D3; Novel ligand; Sensory neurons; TRPV4.

Similar articles

See all similar articles

Cited by 1 article

References

    1. Br J Pharmacol. 2010 Oct;161(3):707-20 - PubMed
    1. Biochem Biophys Res Commun. 2009 Nov 20;389(3):490-4 - PubMed
    1. Annu Rev Neurosci. 2006;29:135-61 - PubMed
    1. J Gen Physiol. 2006 Nov;128(5):509-22 - PubMed
    1. J Gen Physiol. 2009 Mar;133(3):257-62 - PubMed

LinkOut - more resources

Feedback