The purpose is to demonstrate the optical charactering concerning nasopharyngeal tissue of pig by fresh sections and frozen correlating sections with optical coherence tomography (OCT). After being imaged on a fresh specimen, samples are then stored in low temperature refrigerators (-80°C) for one year for the second OCT measurement. The OCT structure of the epithelium, lamina propria, and the basement membrane are still resolvable; the median scattering coefficients and anisotropy factors fitting from OCT images based on the multiple scattering effects for epithelium are 27.6 mm-1 [interquartile range (IQR) 23.6 to 29.3 mm-1] versus 22.5 mm-1 (IQR 20.5 to 24.4 mm-1), 0.86 (IQR 0.81 to 0.9) versus 0.88 (IQR 0.87 to 0.9) for fresh and frozen tissue, respectively; and 10.2 mm-1 (IQR 8.1 to 13.6 mm-1) versus 9.6 mm-1 (IQR 8.1 to 13.8 mm-1), 0.96 (IQR 0.93 to 0.98) versus 0.92 (IQR 0.9 to 0.98) for lamina propria, respectively. The results show that the frozen storage method can be used for OCT research.