MicroRNA-150 promotes cell proliferation, migration, and invasion of cervical cancer through targeting PDCD4

Biomed Pharmacother. 2018 Jan;97:511-517. doi: 10.1016/j.biopha.2017.09.143. Epub 2017 Nov 6.

Abstract

Recent studies have showed that microRNA-150 (miR-150) is up-regulated in various cancers including cervical cancer. However, the specific mechanism of miR-150 in the regulation of cell proliferation, migration and invasion is still unclear. In this study, a total of 150 cervical cancer samples, including 50 cervical cancer tissues, 50 corresponding adjacent non-neoplastic tissues, and 50 serum samples were collected from cervical cancer patients. 50 matched normal tissues and 50 serum samples were collected from the control group. MiR-150 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Programmed cell death protein 4 (PDCD4) was evaluated by qRT-PCR and western blot. Cell migration and invasion were assessed by transwell assays. Proliferative abilities were determined by MTT assays. Luciferase reporter assay was employed to validate the direct target of PDCD4 by miR-150. We found that miR-150 was increased in cervical cancer specimens. In contrast, PDCD4 was decreased in cervical cancer tissues. MiR-150 promoted cell migration, invasion and proliferation through targeting PDCD4. These results collectively indicated that miR-150 might be used as a potential therapeutic biomarker in cervical cancer.

Keywords: Cervical cancer; Invasion; MiR-150; Migration; PDCD4; Proliferation.

MeSH terms

  • Adult
  • Aged
  • Apoptosis Regulatory Proteins / metabolism*
  • Cell Movement / physiology*
  • Cell Proliferation / physiology*
  • Female
  • HeLa Cells
  • Humans
  • MicroRNAs / biosynthesis*
  • Middle Aged
  • Neoplasm Invasiveness / pathology
  • RNA-Binding Proteins / metabolism*
  • Uterine Cervical Neoplasms / metabolism*
  • Uterine Cervical Neoplasms / pathology

Substances

  • Apoptosis Regulatory Proteins
  • MIRN150 microRNA, human
  • MicroRNAs
  • PDCD4 protein, human
  • RNA-Binding Proteins