Application and utility of mass cytometry in vaccine development

FASEB J. 2018 Jan;32(1):5-15. doi: 10.1096/fj.201700325R. Epub 2017 Nov 1.


Mass cytometry enables highly multiplexed profiling of cellular immune responses in limited-volume samples, advancing prospects of a new era of systems immunology. The capabilities of mass cytometry offer expanded potential for deciphering immune responses to infectious diseases and to vaccines. Several studies have used mass cytometry to profile protective immune responses, both postinfection and postvaccination, although no vaccine-development program has yet systematically employed the technology from the outset to inform both candidate design and clinical evaluation. In this article, we review published mass cytometry studies relevant to vaccine development, briefly compare immune profiling by mass cytometry to other systems-level technologies, and discuss some general considerations for deploying mass cytometry in the context of vaccine development.-Reeves, P. M., Sluder, A. E., Raju Paul, S., Scholzen, A., Kashiwagi, S., Poznansky, M. C. Application and utility of mass cytometry in vaccine development.

Keywords: CyTOF; biomarker discovery; flow cytometry.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Antibodies
  • Data Interpretation, Statistical
  • Drug Discovery
  • Flow Cytometry / methods*
  • Flow Cytometry / statistics & numerical data
  • Fluorescent Dyes
  • Gene Expression Profiling
  • Humans
  • Immunity, Cellular
  • Influenza Vaccines / immunology
  • Mice
  • Mice, Inbred C57BL
  • Sequence Analysis, RNA
  • Single-Cell Analysis
  • Systems Biology
  • Vaccines / immunology*


  • Antibodies
  • Fluorescent Dyes
  • Influenza Vaccines
  • Vaccines