Cloning and sequence of a secretory protein induced by growth factors in mouse fibroblasts

Exp Cell Res. 1989 Jan;180(1):266-75. doi: 10.1016/0014-4827(89)90230-9.

Abstract

From a collection of 80 cDNAs representing genes that are induced by serum growth factors in quiescent mouse fibroblasts we have characterized a clone, N51, and demonstrated that it codes for a precursor of a secretory protein. N51 mRNA is rapidly induced by serum, including in the presence of cycloheximide, demonstrating that its induction does not require de novo protein synthesis. The mRNA has a short half-life of approximately 10-15 min and is dramatically prolonged in the presence of protein synthesis inhibitors. Purified growth factors such as platelet-derived growth factor and bombesin are strong inducers of N51 expression. However, epidermal growth factor, fibroblast growth factor, and the tumor promoter tetradecanoyl phorbol acetate have a negligible effect. Transcriptional analysis demonstrates that the increase in N51 mRNA levels after serum stimulation is in part due to the activation of gene transcription.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood
  • Bombesin / pharmacology
  • Cell Line
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chromosome Mapping
  • Cloning, Molecular
  • Culture Media
  • DNA
  • Gene Expression Regulation / drug effects
  • Growth Substances / pharmacology*
  • Intercellular Signaling Peptides and Proteins*
  • Interphase
  • Mice
  • Molecular Sequence Data
  • Platelet-Derived Growth Factor / pharmacology
  • Protein Precursors / biosynthesis
  • Protein Precursors / genetics*
  • RNA, Messenger / genetics
  • Transcription, Genetic

Substances

  • Chemokine CXCL1
  • Chemokines, CXC
  • Culture Media
  • Cxcl1 protein, mouse
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Platelet-Derived Growth Factor
  • Protein Precursors
  • RNA, Messenger
  • DNA
  • Bombesin