Probing Synergistic Effects of DNA Methylation and 2'-β-Fluorination on i-Motif Stability

Chemistry. 2018 Jan 9;24(2):471-477. doi: 10.1002/chem.201704591. Epub 2017 Dec 6.

Abstract

The possible role of DNA i-motif structures in telomere biology and in the transcriptional regulation of oncogene promoter regions is supported by several recent studies. Herein we investigate the effect of four cytidine nucleosides (and combinations thereof) on i-motif structure and stability, namely 2'-deoxycytidine (dC), 2'-deoxy-5-methyl-cytidine (5-Me-dC), 2'-deoxy-2'-fluoro-arabinocytidine (2'F-araC), and 2'-deoxy-2'-fluoro-5-methyl-arabinocytidine (5-Me-2'F-araC). The base pair 5-Me-2'F-araC:2'F-araC produced i-motifs with a pH1/2 ("pKa ") value that closely matches physiological pH (7.34±0.3). NMR analysis of the most stable telomeric sequence (HJ-2) at pH 7.0 indicated that the structure is stabilized by hybrid 5-Me-dC:2'F-araC hemiprotonated base pairs and therefore highlights the significance of the interplay between base and sugar modifications on the stability of i-motif structures.

Keywords: 5-methylcytosine; biological chemistry; epigenetic modifications; nucleosides; telomeric DNA.

MeSH terms

  • Cytarabine / analogs & derivatives*
  • Cytarabine / chemical synthesis
  • Cytarabine / chemistry*
  • Cytidine / analogs & derivatives*
  • Cytidine / chemical synthesis
  • Cytidine / chemistry*
  • DNA / chemistry*
  • DNA Methylation*
  • Halogenation
  • Hydrogen-Ion Concentration
  • Kinetics
  • Molecular Dynamics Simulation
  • Nucleotide Motifs*
  • Telomere / chemistry

Substances

  • Cytarabine
  • Cytidine
  • DNA