Quantitative structural mechanobiology of platelet-driven blood clot contraction

Nat Commun. 2017 Nov 2;8(1):1274. doi: 10.1038/s41467-017-00885-x.


Blood clot contraction plays an important role in prevention of bleeding and in thrombotic disorders. Here, we unveil and quantify the structural mechanisms of clot contraction at the level of single platelets. A key elementary step of contraction is sequential extension-retraction of platelet filopodia attached to fibrin fibers. In contrast to other cell-matrix systems in which cells migrate along fibers, the "hand-over-hand" longitudinal pulling causes shortening and bending of platelet-attached fibers, resulting in formation of fiber kinks. When attached to multiple fibers, platelets densify the fibrin network by pulling on fibers transversely to their longitudinal axes. Single platelets and aggregates use actomyosin contractile machinery and integrin-mediated adhesion to remodel the extracellular matrix, inducing compaction of fibrin into bundled agglomerates tightly associated with activated platelets. The revealed platelet-driven mechanisms of blood clot contraction demonstrate an important new biological application of cell motility principles.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abciximab
  • Actomyosin / metabolism
  • Antibodies, Monoclonal / pharmacology
  • Biomechanical Phenomena
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism*
  • Blood Platelets / pathology
  • Blood Platelets / physiology
  • Cell Adhesion / physiology
  • Fibrin / metabolism*
  • Humans
  • Image Processing, Computer-Assisted
  • Immunoglobulin Fab Fragments / pharmacology
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Glycoprotein GPIIb-IIIa Complex / antagonists & inhibitors
  • Platelet Glycoprotein GPIIb-IIIa Complex / metabolism
  • Pseudopodia / drug effects
  • Pseudopodia / metabolism*
  • Pseudopodia / pathology
  • Pseudopodia / physiology
  • Thrombosis / metabolism*
  • Thrombosis / pathology


  • Antibodies, Monoclonal
  • Immunoglobulin Fab Fragments
  • Platelet Aggregation Inhibitors
  • Platelet Glycoprotein GPIIb-IIIa Complex
  • Fibrin
  • Actomyosin
  • Abciximab