Ultraviolet A Eye Irradiation Ameliorates Atopic Dermatitis via p53 and Clock Gene Proteins in NC/Nga Mice

Photochem Photobiol. 2018 Mar;94(2):378-383. doi: 10.1111/php.12853. Epub 2018 Jan 16.


Atopic dermatitis (AD) is a widespread chronic skin condition that severely affects quality of life and can lead to more serious complications. Although ultraviolet (UV)A eye irradiation can exert various effects on the skin, it is unknown whether UVA can affect AD. To investigate potential associations, we used an NC/Nga mouse model of AD to study the effects of UVA eye irradiation. The eyes of mice were irradiated with a UVA dose of 100 kJ m-2 using a FL20SBLB-A lamp. Our histological data demonstrated that AD symptoms could be ameliorated by UVA eye irradiation. We also observed an increase in the levels of adrenocorticotropic hormone (ACTH), p53 and retinoid X receptor α (RXRα) in mice with UVA-irradiated eyes. In contrast, the levels of thymic stromal lymphopoietin (TSLP), period 2 (PER2) and differentiated embryo chondrocytes 1 (DEC1) protein were decreased in mice treated with UVA irradiation. Furthermore, UVA eye-irradiated mice exhibited reduced DEC1 and RXRα colocalization compared with nonirradiated mice. These results suggested that p53 and various clock gene proteins played important roles in the amelioration of AD symptoms observed after UVA eye irradiation; this technique may have therapeutic applications in AD.

MeSH terms

  • Adrenocorticotropic Hormone / genetics
  • Adrenocorticotropic Hormone / metabolism
  • Animals
  • CLOCK Proteins / genetics
  • CLOCK Proteins / metabolism*
  • Dermatitis, Atopic / radiotherapy*
  • Disease Models, Animal
  • Eye / radiation effects*
  • Male
  • Mice
  • Retinoid X Receptor alpha / genetics
  • Retinoid X Receptor alpha / metabolism
  • Skin / pathology
  • Skin / radiation effects
  • Specific Pathogen-Free Organisms
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*
  • Ultraviolet Therapy / methods*


  • Retinoid X Receptor alpha
  • Trp53 protein, mouse
  • Tumor Suppressor Protein p53
  • Adrenocorticotropic Hormone
  • CLOCK Proteins
  • Clock protein, mouse