Recombinant latcripin 11 of Lentinula edodes C91-3 suppresses the proliferation of various cancer cells

Gene. 2018 Feb 5;642:212-219. doi: 10.1016/j.gene.2017.10.080. Epub 2017 Oct 28.

Abstract

Lentinula edodes C91-3 is an edible mushroom that has demonstrated a remarkable anti-tumor effect in various cancer cells both in vitro and in vivo. In the present study, we report the ability of recombinant thioredoxin-like latcripin 11 (LP-11) of Lentinula edodes C91-3 to suppress the proliferation of various cancer cells. The LP-11 gene of Lentinula edodes C91-3 was cloned in the pET-32a(+) expression vector and expressed in a prokaryotic system. The expressed protein was refolded by gradual dialysis and purified by affinity gel filtration chromatography. The antioxidant activity of LP-11 was tested by 1,1-dipheny l-2-picrylhydrazyl (DPPH) assay. The anti-tumor activity of recombinant LP-11 was tested in eight kinds of tumor cell lines by CCK-8 assay. Recombinant LP-11 significantly suppressed the proliferation of various cancer cells, but not normal human umbilical vein endothelial cells. Human lymphoma U937 cells exhibited the most sensitivity to LP-11 protein. U937 cell apoptosis was assessed by Annexin V staining coupled with flow cytometry, and mitochondrial morphology was analyzed by light and electron microscopy. It was revealed that recombinant LP-11 induced apoptosis in human leukemic monocyte lymphoma U937 cells. Our findings suggest that recombinant LP-11 is a promising agent for the treatment of lymphoma.

Keywords: Antioxidant; Antitumor; Expression; Latcripin-11; Lentinula edodes C91-3.

MeSH terms

  • A549 Cells
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Antioxidants / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Drug Screening Assays, Antitumor
  • Fungal Proteins / genetics
  • Fungal Proteins / pharmacology*
  • HL-60 Cells
  • Hep G2 Cells
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • K562 Cells
  • Mitochondria / drug effects
  • Neoplasms / drug therapy
  • Neoplasms / metabolism*
  • Recombinant Proteins / pharmacology*
  • Shiitake Mushrooms / metabolism*
  • U937 Cells

Substances

  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Fungal Proteins
  • Recombinant Proteins