Proteomic profiling of TGFBI-null mouse corneas reveals only minor changes in matrix composition supportive of TGFBI knockdown as therapy against TGFBI-linked corneal dystrophies

FEBS J. 2018 Jan;285(1):101-114. doi: 10.1111/febs.14321. Epub 2017 Nov 23.

Abstract

TGFBIp is a constituent of the extracellular matrix in many human tissues including the cornea, where it is one of the most abundant proteins expressed. TGFBIp interacts with Type I, II, IV, VI, and XII collagens as well as several members of the integrin family, suggesting it plays an important role in maintaining structural integrity and possibly corneal transparency as well. Significantly, more than 60 point mutations within the TGFBI gene have been reported to result in aberrant TGFBIp folding and aggregation in the cornea, resulting in severe visual impairment and blindness. Several studies have focused on targeting TGFBIp in the cornea as a therapeutic approach to treat TGFBI-linked corneal dystrophies, but the effect of this approach on corneal homeostasis and matrix integrity remained unknown. In the current study, we evaluated the histological and proteomic profiles of corneas from TGFBI-deficient mice as well as potential redundant functions of the paralogous protein POSTN. The absence of TGFBIp in mouse corneas did not grossly affect the collagen scaffold, and POSTN is unable to compensate for loss of TGFBIp. Proteomic comparison of wild-type and TGFBI-/- mice revealed 11 proteins were differentially regulated, including Type VI and XII collagens. However, as these alterations did not manifest at the macroscopic and behavioral levels, these data support partial or complete TGFBI knockdown as a potential therapy against TGFBI-linked corneal dystrophies. Lastly, in situ hybridization verified TGFBI mRNA in the epithelial cells but not in other cell types, supportive of a therapy directed specifically at this lineage.

Keywords: ECM; POSTN; iTRAQ; LC-MS/MS; TGFBIp; cornea.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Animals
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism
  • Cornea / metabolism*
  • Cornea / ultrastructure
  • Corneal Dystrophies, Hereditary / genetics
  • Corneal Dystrophies, Hereditary / metabolism*
  • Corneal Dystrophies, Hereditary / therapy
  • Epithelial Cells / metabolism
  • Extracellular Matrix / metabolism*
  • Female
  • Gene Expression
  • Humans
  • Male
  • Mice
  • Mice, Knockout
  • Proteomics / methods*
  • Transforming Growth Factor beta / deficiency*
  • Transforming Growth Factor beta / genetics

Substances

  • Cell Adhesion Molecules
  • Postn protein, mouse
  • Transforming Growth Factor beta

Associated data

  • PDB/5NV6
  • SWISSPROT/Q15582
  • SWISSPROT/P40124
  • SWISSPROT/P07356
  • SWISSPROT/Q60847
  • SWISSPROT/Q02788
  • SWISSPROT/P50608
  • SWISSPROT/Q9WVH9
  • SWISSPROT/P14602
  • SWISSPROT/Q9Z331
  • SWISSPROT/Q9JK53
  • SWISSPROT/Q9R1P1
  • SWISSPROT/P07724