Ionic liquid mediated stereoselective synthesis of alanine linked hybrid quinazoline-4(3H)-one derivatives perturbing the malarial reductase activity in folate pathway

Bioorg Med Chem. 2017 Dec 15;25(24):6635-6646. doi: 10.1016/j.bmc.2017.10.041. Epub 2017 Oct 31.


Grimmel's method was optimized as well as modified leading to the cyclization and incorporation of alanine linked sulphonamide in 4-quinazolin-(3H)-ones. Further, the generation of heterocyclic motif at position-3 of 4-quinazolinones was explored by synthesis of imines, which unfortunately led to an isomeric mixture of stereoisomers. The hurdle of diastereomers encountered on the path was eminently rectified by development of new rapid and reproducible methodology involving the use of imidazolium based ionic liquid as solvents as well as catalyst for cyclization as well as synthesis of imines in situ at position-3 leading to procurement of single E-isomer as the target hybrid heterocyclic molecules. The purity and presence of single isomer was also confirmed by HPLC and spectroscopic techniques. Further, the synthesized sulphonamide linked 4-quinazolin-(3H)-ones hybrids were screened for their antimalarial potency rendering potent entities (4b, 4c, 4 l, 4 t and 4u). The active hybrids were progressively screened for enzyme inhibitory efficacy against presumed receptor Pf-DHFR and h-DHFR computationally as well as in vitro, proving their potency as dihydrofolate reductase inhibitors. The ADME properties of these active molecules were also predicted to enhance the knowhow of the oral bioavailability, indicating good bioavailability of the active entities.

Keywords: 1-n-butyl-3-methylimidazolium tetrafluoroborate; Amino acid; Cytotoxicity; Maestro; Pharmacokinetic properties; [Bmim][BF(4)]-H(2)O.

MeSH terms

  • Alanine / chemistry
  • Alanine / pharmacology*
  • Animals
  • Antimalarials / chemical synthesis
  • Antimalarials / chemistry
  • Antimalarials / pharmacology*
  • Caco-2 Cells
  • Catalysis
  • Chlorocebus aethiops
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Folic Acid / metabolism
  • Humans
  • Ionic Liquids / chemistry
  • Models, Molecular
  • Molecular Structure
  • Parasitic Sensitivity Tests
  • Plasmodium falciparum / drug effects*
  • Quinazolinones / chemical synthesis
  • Quinazolinones / chemistry
  • Quinazolinones / pharmacology*
  • Stereoisomerism
  • Structure-Activity Relationship
  • Tetrahydrofolate Dehydrogenase / metabolism*
  • Vero Cells


  • Antimalarials
  • Enzyme Inhibitors
  • Ionic Liquids
  • Quinazolinones
  • 4-hydroxyquinazoline
  • Folic Acid
  • Tetrahydrofolate Dehydrogenase
  • Alanine