Flow Cytometry of B-Cell Neoplasms

Clin Lab Med. 2017 Dec;37(4):697-723. doi: 10.1016/j.cll.2017.07.001. Epub 2017 Aug 31.


Flow cytometric evaluation is considered a standard ancillary study for the diagnosis of most B-cell lymphoproliferative disorders. Establishing a neoplastic B-cell population depends on identification of light chain restriction or lack of light chain expression in mature neoplasms and demonstration of aberrant antigen expression in both immature and mature neoplasms, as compared with normal counterparts. The immunophenotypes of the most common B-cell neoplasms are herein described, with an emphasis on their immunophenotypic differential diagnosis and prognostic and therapeutic implications.

Keywords: Aggressive B-cell lymphomas; Chronic lymphoproliferative disorders; Flow cytometry; Immunophenotypic aberrancy; Light chains/clonality; Small B-cell lymphomas.

Publication types

  • Review

MeSH terms

  • Flow Cytometry*
  • Humans
  • Immunophenotyping
  • Lymphoma, B-Cell* / classification
  • Lymphoma, B-Cell* / diagnosis