OsPRX2 contributes to stomatal closure and improves potassium deficiency tolerance in rice

Xiaohui Mao; Yanmei Zheng; Kaizhuan Xiao; Yidong Wei; Yongsheng Zhu; Qiuhua Cai; Liping Chen; Huaan Xie; Jianfu Zhang

doi:10.1016/j.bbrc.2017.11.045

OsPRX2 contributes to stomatal closure and improves potassium deficiency tolerance in rice

Biochem Biophys Res Commun. 2018 Jan 1;495(1):461-467. doi: 10.1016/j.bbrc.2017.11.045. Epub 2017 Nov 8.

Authors

Xiaohui Mao¹, Yanmei Zheng², Kaizhuan Xiao², Yidong Wei², Yongsheng Zhu², Qiuhua Cai², Liping Chen², Huaan Xie³, Jianfu Zhang⁴

Affiliations

¹ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou Branch, National Center of Rice Improvement of China, Fuzhou 350003, China; National Engineering Laboratory of Rice, Fuzhou 350003, China; South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China.
² Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou Branch, National Center of Rice Improvement of China, Fuzhou 350003, China; National Engineering Laboratory of Rice, Fuzhou 350003, China; South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China.
³ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou Branch, National Center of Rice Improvement of China, Fuzhou 350003, China; National Engineering Laboratory of Rice, Fuzhou 350003, China; South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China. Electronic address: huaanxie@163.com.
⁴ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou Branch, National Center of Rice Improvement of China, Fuzhou 350003, China; National Engineering Laboratory of Rice, Fuzhou 350003, China; South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China. Electronic address: jianfzhang@163.com.

PMID: 29128357
DOI: 10.1016/j.bbrc.2017.11.045

Abstract

Peroxiredoxins (Prxs) which are thiol-based peroxidases have been implicated in the toxic reduction and intracellular concentration regulation of hydrogen peroxide. In Arabidopsis thaliana At2-CysPrxB (At5g06290) has been demonstrated to be essential in maintaining the water-water cycle for proper H₂O₂ scavenging. Although the mechanisms of 2-Cys Prxs have been extensively studied in Arabidopsis thaliana, the function of 2-Cys Prxs in rice is unclear. In this study, a rice homologue gene of At2-CysPrxB, OsPRX2 was investigated aiming to characterize the effect of 2-Cys Prxs on the K⁺-deficiency tolerance in rice. We found that OsPRX2 was localized in the chloroplast. Overexpressed OsPRX2 causes the stomatal closing and K⁺-deficiency tolerance increasing, while knockout of OsPRX2 lead to serious defects in leaves phenotype and the stomatal opening under the K⁺-deficiency tolerance. Detection of K⁺ accumulation, antioxidant activity of transgenic plants under the starvation of potassium, further confirmed that OsPRX2 is a potential target for engineering plants with improved potassium deficiency tolerance.

Keywords: 2-Cys peroxiredoxins; Potassium; Rice; Stomatal closure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Gene Expression Regulation, Plant
Oryza / anatomy & histology
Oryza / genetics
Oryza / metabolism*
Oryza / ultrastructure
Peroxiredoxins / analysis
Peroxiredoxins / genetics
Peroxiredoxins / metabolism*
Phylogeny
Plant Proteins / analysis
Plant Proteins / genetics
Plant Proteins / metabolism*
Plant Stomata / anatomy & histology
Plant Stomata / genetics
Plant Stomata / metabolism*
Plant Stomata / ultrastructure
Plants, Genetically Modified / anatomy & histology
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Plants, Genetically Modified / ultrastructure
Potassium / metabolism*
Up-Regulation

Substances

Plant Proteins
Peroxiredoxins
Potassium