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. 2018 Jan 2;455:10-13.
doi: 10.1016/j.carres.2017.10.022. Epub 2017 Nov 4.

Tandem Mass Spectrometry of Fucoidan-Derived Fragments, Labeled With Heavy-Oxygen

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Tandem Mass Spectrometry of Fucoidan-Derived Fragments, Labeled With Heavy-Oxygen

Stanislav D Anastyuk et al. Carbohydr Res. .

Abstract

A procedure for the partial depolymerization of sulfated fucans and selective labeling with 18O was developed. A tandem electrospray ionization mass spectrometry (ESI MS/MS) was applied for the direct analysis of mixtures of structurally-different oligosaccharides, derived from the fucoidans of known structure. The presence of label allowed unambiguous distinguishing between the fragment ions of 0,2X0-type at m/z 287 and 2,4A-type at m/z 285, since 18O at the reducing end gave +2 mass shifting. Thus, ESI MS/MS was able to detect (1,2)-type of linkage in disaccharides from the fucoidan of brown alga S. cichorioides for the first time. It was also discovered that 2,4A-type fragments in 4-linked disaccharides that were incorrectly assigned to 0,2X-type previously, suggested, probably, substitution at C-4 in mono- and disaccharide fragments, derived from the fucoidan of the brown alga F. evanescens.

Keywords: Derivatization; Fucoidan; Heavy-oxygen; Labeling; Mass spectrometry.

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