Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography

Methods Mol Biol. 2018;1673:363-370. doi: 10.1007/978-1-4939-7309-5_27.

Abstract

Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.

Keywords: Accessory gene regulator; High performance liquid chromatography; Staphylococcal quorum sensing inhibitors; Staphylococcus aureus; õ-Toxin.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Hemolysin Proteins / metabolism*
  • Quorum Sensing*
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*
  • Staphylococcus epidermidis / growth & development
  • Staphylococcus epidermidis / metabolism*

Substances

  • Hemolysin Proteins