Human Dendritic Cells with Th2-Polarizing Capacity: Analysis Using Label-Free Quantitative Proteomics

Int Arch Allergy Immunol. 2017;174(3-4):170-182. doi: 10.1159/000479512. Epub 2017 Nov 10.


Background: Dendritic cells (DCs) are the sentinels of the immune system. Upon recognition of a pathogen, they mature and migrate to draining lymph nodes to prime and polarize T cell responses. Although it is known that helminths and helminth-derived molecules condition DCs to polarize T helper (Th) cells towards Th2, the underlying mechanisms remain incompletely understood.

Objectives: The aim of this study was to conduct a proteome analysis of helminth antigen-stimulated DCs in order to gain more insight into the cellular processes associated with their ability to polarize immune responses.

Methods: We analyzed the maturation and polarization of monocyte-derived DCs from 9 donors at 2 different time points after stimulation with different Th1- and Th2-polarizing pathogen-derived molecules. The samples were measured using liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry for relative quantitation.

Results: Lipopolysaccharide-induced maturation promoted the expression of proteins related to metabolic, cellular, and immune system processes. Th1-polarizing DCs, conditioned by IFN-γ during maturation, displayed accelerated maturation by differentially expressing cytoskeletal proteins and proteins involved in immune regulation. The stimulation of DCs with soluble egg antigens and omega-1 derived from Schistosoma mansoni, which are both Th2-inducing stimuli, increased 60S acidic ribosomal protein P2, and vesicle amine transferase 1 while decreasing the expression of proteins related to antigen processing and presentation.

Conclusion: Our data indicate that not only proteins involved in the interaction between T cells and DCs at the level of the immunological synapse, but also those related to cellular metabolism and stress, may promote Th2 polarization.

Keywords: Dendritic cells; Helminths; Omega-1; Proteome; T helper 2 responses.

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigens, Helminth / immunology
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Egg Proteins / immunology
  • Humans
  • Immune Evasion
  • Interferon-gamma / immunology
  • Lipopolysaccharides / immunology
  • Proteomics
  • Schistosoma mansoni / immunology*
  • Schistosomiasis mansoni / immunology*
  • Th1 Cells / immunology*
  • Th2 Cells / immunology*


  • Antigens, Helminth
  • Egg Proteins
  • Lipopolysaccharides
  • omega-1 antigen, Schistosoma mansoni
  • Interferon-gamma