A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

Sarah H Hewitt; Jonathan Parris; Romain Mailhot; Stephen J Butler

doi:10.1039/c7cc05887j

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

Chem Commun (Camb). 2017 Nov 23;53(94):12626-12629. doi: 10.1039/c7cc05887j.

Authors

Sarah H Hewitt¹, Jonathan Parris, Romain Mailhot, Stephen J Butler

Affiliation

¹ Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK. s.j.butler@lboro.ac.uk.

PMID: 29131206
DOI: 10.1039/c7cc05887j

Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg²⁺ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

MeSH terms

Adenosine Diphosphate / metabolism*
Adenosine Triphosphate / metabolism*
Coordination Complexes / chemistry*
Europium / chemistry*
Luminescent Measurements / methods*
Phosphotransferases / analysis*
Phosphotransferases / metabolism*

Substances

Coordination Complexes
Europium
Adenosine Diphosphate
Adenosine Triphosphate
Phosphotransferases