Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae

Fidelis Enyinnaya; Patricia Cruz; Mark P Buttner; Chad Cross; David R Woodard

doi:10.4155/fsoa-2017-0095

Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae

Future Sci OA. 2017 Oct 5;3(4):FSO245. doi: 10.4155/fsoa-2017-0095. eCollection 2017 Nov.

Authors

Fidelis Enyinnaya^{1

2

1

2}, Patricia Cruz^{1

1}, Mark P Buttner^{1

1}, Chad Cross^{1

3

1

3}, David R Woodard^{1

1}

Affiliations

¹ Department of Environmental & Occupational Health, School of Community Health Sciences, University of Nevada, Las Vegas, 4505 S. Maryland Parkway, Box 453064, Las Vegas, NV 89154-3064, USA.
² Department of Surgical Critical Care, Hartford Hospital, 80 Seymour Street Hartford, CT 06102, USA.
³ Department of Radiation Oncology, School of Medicine, University of Nevada, Las Vegas, 4505 S. Maryland Parkway, Box 453070, Las Vegas, NV 89154-3070, USA.

Abstract

Aim: Carbapenems are antibiotics reserved for treatment of severe infections. Carbapenem antimicrobial susceptibility testing profiles were determined in a population of Klebsiella pneumoniae, and their resistance assessed based on previous and current Clinical and Laboratory Standards Institute criteria.

Materials & methods: Isolates were examined using an automated antimicrobial susceptibility testing method, and real time polymerase chain reaction to detect the resistance (blaKPC) gene.

Results: The prevalence of blaKPC gene was 45/54 (83.3%). Five isolates that were susceptible under the previous criteria changed to nonsusceptible with the current standards. The overall difference in susceptibility between the standards was 8%.

Conclusion: This study shows that the current Clinical and Laboratory Standards Institute criteria may not offer additional benefits in the fight against carbapenem-resistant Enterobacteriaceae.

Keywords: Clinical and Laboratory Standards Institute; Klebsiella pneumoniae; antibiotics; antimicrobial stewardship; carbapenem; carbapenem resistance.