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. 2018 Feb;163(2):545-548.
doi: 10.1007/s00705-017-3618-4. Epub 2017 Nov 14.

Rapid Visual Detection of Lily Mottle Virus Using a Loop-Mediated Isothermal Amplification Method

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Rapid Visual Detection of Lily Mottle Virus Using a Loop-Mediated Isothermal Amplification Method

Binan Zhao et al. Arch Virol. .

Abstract

Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

Keywords: LAMP; Potyvirus; ornamental plant; virus detection.

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