Red-emitting BSA-stabilized copper nanoclusters acted as a sensitive probe for fluorescence sensing and visual imaging detection of rutin

Benqian Wang; Rijun Gui; Hui Jin; Weijie He; Zonghua Wang

doi:10.1016/j.talanta.2017.08.102

Red-emitting BSA-stabilized copper nanoclusters acted as a sensitive probe for fluorescence sensing and visual imaging detection of rutin

Talanta. 2018 Feb 1:178:1006-1010. doi: 10.1016/j.talanta.2017.08.102. Epub 2017 Sep 1.

Authors

Benqian Wang¹, Rijun Gui², Hui Jin¹, Weijie He¹, Zonghua Wang³

Affiliations

¹ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
² College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China. Electronic address: guirijun@163.com.
³ College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China. Electronic address: wangzonghua@qdu.edu.cn.

PMID: 29136788
DOI: 10.1016/j.talanta.2017.08.102

Abstract

In this article, red-emitting copper nanoclusters (Cu NCs) were prepared in the matrix of bovine serum albumin (BSA) through using N₂H₄·H₂O as a reducing agent and BSA as a stabilizer. In the aqueous suspension of BSA-Cu NCs, the addition of rutin enabled the combination of rutin with BSA around Cu NCs via hydrogen bond and electrostatic interactions between functional groups of BSA and rutin. The approaching of rutin to Cu NCs induced that the fluorescence (FL) of Cu NCs was quenched by rutin due to the electron transfer from Cu NCs to rutin. Under the optimal conditions, the FL of Cu NCs was linearly decreased with the increase of rutin concentration from 0.1 to100 μM, together with a low detection limit of ca. 0.02μM. The FL quenching responses of Cu NCs were highly selective and sensitive towards rutin, over potential interferents in real human serum samples. Based on BSA-Cu NCs, this as-proposed FL probe exhibited a superior capability of rutin detection in real human serum samples. Significantly, this probe of rutin could realize the FL sensing of rutin and the naked-eye visual FL imaging detection of rutin in aqueous solution and on filter paper.

Keywords: Bovine serum albumin; Copper nanoclusters; Fluorescence; Imaging; Rutin.

MeSH terms

Biosensing Techniques / methods*
Color
Copper / chemistry*
Electron Transport
Filtration
Fluorescent Dyes / chemistry*
Models, Molecular
Nanostructures / chemistry*
Optical Imaging / methods*
Paper
Protein Conformation
Rutin / analysis*
Serum Albumin, Bovine / chemistry*
Water / chemistry

Substances

Fluorescent Dyes
Water
Serum Albumin, Bovine
Rutin
Copper